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Ective.%20Science%202000,%20287:1960?1964.%202.%20Hopkins%201-phenyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole1-phenyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole AL, Groom CR: The druggable genome. Nat Rev Drug Discov 2002, 1:727?30. 3. Stone LS, Molliver DC: In search of analgesia: emerging roles of GPCRs in pain. Mol Interv 2009, 9:234?51. 4. Julius D, Basbaum AI: Molecular mechanisms of nociception. Nature 2001, 413:203?10. 5. Tappe-Theodor A, Constantin CE, Tegeder I, Lechner SG, Langeslag M, Lepcynzsky P, Wirotanseng RI, Kurejova M, Agarwal N, Nagy G, et al: Galpha (q/11) signaling tonically modulates nociceptor function and contributes to activity-dependent sensitization. Pain 2012, 153:184?96. 6. Wettschureck N, Offermanns S: Mammalian G proteins and their cell type specific functions. Physiol Rev 2005, 85:1159?204. 7. Han SK, Mancino V, Simon MI: Phospholipase Cbeta 3 mediates the scratching response activated by the histamine H1 receptor on C-fiber nociceptive neurons. Neuron 2006, 52:691?03. 8. Wettschureck N, Rutten H, Zywietz A, Gehring D, Wilkie TM, Chen J, Chien KR, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23410069 Offermanns S: Absence of pressure overload induced myocardial hypertrophy after conditional inactivation of Galphaq/Galpha11 in cardiomyocytes. Nat Med 2001, 7:1236?240. 9. Strathmann M, Simon MI: G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates. Proc Natl Acad Sci USA 1990, 87:9113?117.Wirotanseng et al. Molecular Pain PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20618955 2013, 9:54 http://www.molecularpain.com/content/9/1/Page 6 of10. Berstein G, Blank JL, Smrcka AV, Higashijima T, Sternweis PC, Exton JH, Ross EM: Reconstitution of agonist-stimulated phosphatidylinositol 4,5bisphosphate hydrolysis using purified m1 muscarinic receptor, Gq/11, and phospholipase C-beta 1. J Biol Chem 1992, 267:8081?088. 11. Offermanns S, Heiler E, Spicher K, Schultz 4-Bromopicolinaldehyde G: Gq and G11 are concurrently activated by bombesin and vasopressin in Swiss 3T3 cells. FEBS Lett 1994, 349:201?04. 12. Wange RL, Smrcka AV, Sternweis PC, Exton JH: Photoaffinity labeling of two rat liver plasma membrane proteins with [32P]gamma-azidoanilido GTP in response to vasopressin. Immunologic identification as alpha subunits of the Gq class of G proteins. J Biol Chem 1991, 266:11409?1412. 13. Wu DQ, Lee CH, Rhee SG, Simon MI: Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells. J Biol Chem 1992, 267:1811?817. 14. Offermanns S, Hashimoto K, Watanabe M, Sun W, Kurihara H, Thompson RF, Inoue Y, Kano M, Simon MI: Impaired motor coordination and persistent multiple climbing fiber innervation of cerebellar Purkinje cells in mice lacking Galphaq. Proc Natl Acad Sci U S A 1997, 94:14089?4094. 15. Hartmann J, Blum R, Kovalchuk Y, Adelsberger H, Kuner R, Durand GM, Miyata M, Kano M, Offermanns S, Konnerth A: Distinct roles of Galpha(q) and Galpha11 for Purkinje cell signaling and motor behavior. J Neurosci 2004, 24:5119?130. 16. Kleppisch T, Voigt V, Allmann R, Offermanns S: G(alpha)q-deficient mice lack metabotropic glutamate receptor-dependent long-term depression but show normal long-term potentiation in the hippocampal CA1 region. J Neurosci 2001, 21:4943?948. 17. Offermanns S, Toombs CF, Hu YH, Simon MI: Defective platelet activation in G alpha(q)-deficient mice. Nature 1997, 389:183?86. 18. Haley JE, Abogadie FC, Delmas P, Dayrell M, Vallis Y, Milligan G, Caulfield MP, 5-tetramethyl-1 Brown DA, Buckley NJ: The alpha subunit of Gq contributes to muscarinic inhibition of the M-type potassium current in sympathetic neurons. J Neurosci 1998, 18:4521?531. 19. Haley JE, Delmas P, Offermanns S,.